A.2. Supported Feature Formats

A.2.1. DASGFF

DAS format is for output only. It conforms to the output of a DAS Distributed Annotation System version 1.53 annotation server. DAS is an XML format for sequence data. For more information see:

http://www.acedb.org/
<?xml version="1.0" standalone="no"?>
<!DOCTYPE DASGFF SYSTEM "http://www.biodas.org/dtd/dasgff.dtd">
<DASGFF>
  <GFF version="1.0" href="url">
    <SEGMENT id="X65921" start="1" stop="2017"
                version="0.0">
      <FEATURE id="X65921.EMBL.1">
         <TYPE id="SO:2000061" category="other" reference="no" subparts="no">databank_entry</TYPE>
         <METHOD id="EMBL"></METHOD>
         <START>1</START>
         <END>2016</END>
         <SCORE>0.000000</SCORE>
         <ORIENTATION>+</ORIENTATION>
         <PHASE>-</PHASE>
         <NOTE>organism:Homo sapiens</NOTE>
         <NOTE>mol_type:genomic DNA</NOTE>
         <NOTE>clone_lib:CML cosmid</NOTE>
         <NOTE>clone:15.1</NOTE>
         <NOTE>db_xref:taxon:9606</NOTE>
      </FEATURE>
      <FEATURE id="X65921.EMBL.2">
         <TYPE id="SO:0000234" category="transcription" reference="no" subparts="no">mRNA</TYPE>
         <METHOD id="EMBL"></METHOD>
         <START>408</START>
         <END>504</END>
         <SCORE>0.000000</SCORE>
         <ORIENTATION>+</ORIENTATION>
         <PHASE>-</PHASE>
         <NOTE>gene:fau 1</NOTE>
      </FEATURE>
      <FEATURE id="X65921.EMBL.2">
         <TYPE id="SO:0000234" category="transcription" reference="no" subparts="no">mRNA</TYPE>
         <METHOD id="EMBL"></METHOD>
         <START>774</START>
         <END>856</END>
         <SCORE>0.000000</SCORE>
         <ORIENTATION>+</ORIENTATION>
         <PHASE>-</PHASE>
      </FEATURE>
      <FEATURE id="X65921.EMBL.2">
         <TYPE id="SO:0000234" category="transcription" reference="no" subparts="no">mRNA</TYPE>
         <METHOD id="EMBL"></METHOD>
         <START>951</START>
         <END>1095</END>
         <SCORE>0.000000</SCORE>
         <ORIENTATION>+</ORIENTATION>
         <PHASE>-</PHASE>
      </FEATURE>
      <FEATURE id="X65921.EMBL.2">
         <TYPE id="SO:0000234" category="transcription" reference="no" subparts="no">mRNA</TYPE>
         <METHOD id="EMBL"></METHOD>
         <START>1557</START>
         <END>1612</END>
         <SCORE>0.000000</SCORE>
         <ORIENTATION>+</ORIENTATION>
         <PHASE>-</PHASE>
      </FEATURE>
      <FEATURE id="X65921.EMBL.2">
         <TYPE id="SO:0000234" category="transcription" reference="no" subparts="no">mRNA</TYPE>
         <METHOD id="EMBL"></METHOD>
         <START>1787</START>
         <END>1912</END>
         <SCORE>0.000000</SCORE>
         <ORIENTATION>+</ORIENTATION>
         <PHASE>-</PHASE>
      </FEATURE>
      <FEATURE id="X65921.EMBL.3">
         <TYPE id="SO:0000147" category="transcription" reference="no" subparts="no">exon</TYPE>
         <METHOD id="EMBL"></METHOD>
         <START>408</START>
         <END>504</END>
         <SCORE>0.000000</SCORE>
         <ORIENTATION>+</ORIENTATION>
         <PHASE>-</PHASE>
         <NOTE>number:1</NOTE>
      </FEATURE>
      <FEATURE id="X65921.EMBL.4">
         <TYPE id="SO:0000188" category="transcription" reference="no" subparts="no">intron</TYPE>
         <METHOD id="EMBL"></METHOD>
         <START>505</START>
         <END>773</END>
         <SCORE>0.000000</SCORE>
         <ORIENTATION>+</ORIENTATION>
         <PHASE>-</PHASE>
         <NOTE>number:1</NOTE>
      </FEATURE>
      <FEATURE id="X65921.EMBL.5">
         <TYPE id="SO:0000147" category="transcription" reference="no" subparts="no">exon</TYPE>
         <METHOD id="EMBL"></METHOD>
         <START>774</START>
         <END>856</END>
         <SCORE>0.000000</SCORE>
         <ORIENTATION>+</ORIENTATION>
         <PHASE>-</PHASE>
         <NOTE>number:2</NOTE>
      </FEATURE>
      <FEATURE id="X65921.EMBL.6">
         <TYPE id="SO:0000316" category="translation" reference="no" subparts="no">CDS</TYPE>
         <METHOD id="EMBL"></METHOD>
         <START>782</START>
         <END>856</END>
         <SCORE>0.000000</SCORE>
         <ORIENTATION>+</ORIENTATION>
         <PHASE>-</PHASE>
         <NOTE>gene:fau 1</NOTE>
         <NOTE>db_xref:GDB:135476</NOTE>
         <NOTE>db_xref:GOA:P62861</NOTE>
         <NOTE>db_xref:HGNC:3597</NOTE>
         <NOTE>db_xref:HSSP:1GJZ</NOTE>
         <NOTE>db_xref:InterPro:IPR006846</NOTE>
         <NOTE>db_xref:UniProtKB/Swiss-Prot:P35544</NOTE>
         <NOTE>protein_id:CAA46714.1</NOTE>
         <NOTE>translation:MQLFVRAQELHTFEVTGQETVAQIKAHVASLEGIAPEDQVVLLAGAPLEDEATLGQCGVEALTTLEVAGRMLGGKVHGSLARAGKVRGQTPKVAKQEKKKKKTGRAKRRMQYNRRFVNVVPTFGKKKGPNANS</NOTE>
      </FEATURE>
      <FEATURE id="X65921.EMBL.6">
         <TYPE id="SO:0000316" category="translation" reference="no" subparts="no">CDS</TYPE>
         <METHOD id="EMBL"></METHOD>
         <START>951</START>
         <END>1095</END>
         <SCORE>0.000000</SCORE>
         <ORIENTATION>+</ORIENTATION>
         <PHASE>-</PHASE>
      </FEATURE>
      <FEATURE id="X65921.EMBL.6">
         <TYPE id="SO:0000316" category="translation" reference="no" subparts="no">CDS</TYPE>
         <METHOD id="EMBL"></METHOD>
         <START>1557</START>
         <END>1612</END>
         <SCORE>0.000000</SCORE>
         <ORIENTATION>+</ORIENTATION>
         <PHASE>-</PHASE>
      </FEATURE>
      <FEATURE id="X65921.EMBL.6">
         <TYPE id="SO:0000316" category="translation" reference="no" subparts="no">CDS</TYPE>
         <METHOD id="EMBL"></METHOD>
         <START>1787</START>
         <END>1912</END>
         <SCORE>0.000000</SCORE>
         <ORIENTATION>+</ORIENTATION>
         <PHASE>-</PHASE>
      </FEATURE>
      <FEATURE id="X65921.EMBL.7">
         <TYPE id="SO:0000188" category="transcription" reference="no" subparts="no">intron</TYPE>
         <METHOD id="EMBL"></METHOD>
         <START>857</START>
         <END>950</END>
         <SCORE>0.000000</SCORE>
         <ORIENTATION>+</ORIENTATION>
         <PHASE>-</PHASE>
         <NOTE>number:2</NOTE>
      </FEATURE>
      <FEATURE id="X65921.EMBL.8">
         <TYPE id="SO:0000147" category="transcription" reference="no" subparts="no">exon</TYPE>
         <METHOD id="EMBL"></METHOD>
         <START>951</START>
         <END>1095</END>
         <SCORE>0.000000</SCORE>
         <ORIENTATION>+</ORIENTATION>
         <PHASE>-</PHASE>
         <NOTE>number:3</NOTE>
      </FEATURE>
      <FEATURE id="X65921.EMBL.9">
         <TYPE id="SO:0000188" category="transcription" reference="no" subparts="no">intron</TYPE>
         <METHOD id="EMBL"></METHOD>
         <START>1096</START>
         <END>1556</END>
         <SCORE>0.000000</SCORE>
         <ORIENTATION>+</ORIENTATION>
         <PHASE>-</PHASE>
         <NOTE>number:3</NOTE>
      </FEATURE>
      <FEATURE id="X65921.EMBL.10">
         <TYPE id="SO:0000147" category="transcription" reference="no" subparts="no">exon</TYPE>
         <METHOD id="EMBL"></METHOD>
         <START>1557</START>
         <END>1612</END>
         <SCORE>0.000000</SCORE>
         <ORIENTATION>+</ORIENTATION>
         <PHASE>-</PHASE>
         <NOTE>number:4</NOTE>
      </FEATURE>
      <FEATURE id="X65921.EMBL.11">
         <TYPE id="SO:0000188" category="transcription" reference="no" subparts="no">intron</TYPE>
         <METHOD id="EMBL"></METHOD>
         <START>1613</START>
         <END>1786</END>
         <SCORE>0.000000</SCORE>
         <ORIENTATION>+</ORIENTATION>
         <PHASE>-</PHASE>
         <NOTE>number:4</NOTE>
      </FEATURE>
      <FEATURE id="X65921.EMBL.12">
         <TYPE id="SO:0000147" category="transcription" reference="no" subparts="no">exon</TYPE>
         <METHOD id="EMBL"></METHOD>
         <START>1787</START>
         <END>1912</END>
         <SCORE>0.000000</SCORE>
         <ORIENTATION>+</ORIENTATION>
         <PHASE>-</PHASE>
         <NOTE>number:5</NOTE>
      </FEATURE>
      <FEATURE id="X65921.EMBL.13">
         <TYPE id="SO:0000551" category="transcription" reference="no" subparts="no">polyA_signal_sequence</TYPE>
         <METHOD id="EMBL"></METHOD>
         <START>1938</START>
         <END>1943</END>
         <SCORE>0.000000</SCORE>
         <ORIENTATION>+</ORIENTATION>
         <PHASE>-</PHASE>
      </FEATURE>
    </SEGMENT>
  </GFF>
</DASGFF>

A.2.2. EMBL, GenBank, DDBJ

Feature table format used by the GenBank, EMBL and DDBJ nucleic acid sequence databanks.

GenBank / DDBJ has a different header record and lacks line codes but otherwise has the same content as EMBL. On output users must choose whether to write "embl" or "genbank" format.

The International Nucleotide Sequence Database Collaboration, comprised of the European Molecular Biology Laboratory (EMBL), DNA DataBank of Japan (DDBJ), and GenBank at the National Center for Biotechnology Information, devised a common feature table format and common standards for annotation practice. The feature table describes the roles and locations of higher order sequence domains and elements within the genome of an organism. It provides an extensive vocabulary for describing features in a flexible framework for manipulating them. The feature table documentation represents the shared rules that allow the three databases to exchange data on a daily basis. For further information see:

http://www.ebi.ac.uk/embl/Documentation/FT_definitions/feature_table.html
FH   Key             Location/Qualifiers
FH
FT   source          1..2016
FT                   /organism="Homo sapiens"
FT                   /mol_type="genomic DNA"
FT                   /clone_lib="CML cosmid"
FT                   /clone="15.1"
FT                   /db_xref="taxon:9606"
FT   mRNA            join(408..504,774..856,951..1095,1557..1612,1787..>1912)
FT                   /gene="fau 1"
FT   exon            408..504
FT                   /number=1
FT   intron          505..773
FT                   /number=1
FT   exon            774..856
FT                   /number=2
FT   CDS             join(782..856,951..1095,1557..1612,1787..1912)
FT                   /gene="fau 1"
FT                   /db_xref="GDB:135476"
FT                   /db_xref="GOA:P62861"
FT                   /db_xref="HGNC:3597"
FT                   /db_xref="HSSP:1GJZ"
FT                   /db_xref="InterPro:IPR006846"
FT                   /db_xref="UniProtKB/Swiss-Prot:P35544"
FT                   /protein_id="CAA46714.1"
FT                   /translation="MQLFVRAQELHTFEVTGQETVAQIKAHVASLEGIAPEDQVVLLAG
FT                   APLEDEATLGQCGVEALTTLEVAGRMLGGKVHGSLARAGKVRGQTPKVAKQEKKKKKTG
FT                   RAKRRMQYNRRFVNVVPTFGKKKGPNANS"
FT   intron          857..950
FT                   /number=2
FT   exon            951..1095
FT                   /number=3
FT   intron          1096..1556
FT                   /number=3
FT   exon            1557..1612
FT                   /number=4
FT   intron          1613..1786
FT                   /number=4
FT   exon            1787..>1912
FT                   /number=5
FT   polyA_signal    1938..1943

A.2.3. GFF3

The General Feature Format (GFF) is an extension developed by Lincoln Stein and the Sequence Ontology project of the previous GFF2 format developed at the Sanger Institute to describe genes and other features associated with DNA, RNA and protein sequences. GFF describes each feature with a name, start position and end position (or "NSE") along with extended annotation. Feature names are required to be names of terms in the Sequence Ontology (SO). The format also provides support for groups of features. GFF format is normally used to hold pure feature information only, but can hold the sequence immediately after the feature table. A complete specification of the format is available at:

http://www.sequenceontology.org/gff3.shtml
##gff-version 3
##sequence-region X65921 1 2016
#!Date 2009-07-30
#!Type DNA
#!Source-version EMBOSS 6.1.0
X65921	EMBL	databank_entry	1	2016	0.000	+	.	ID="X65921.1";organism="Homo sapiens";mol_type="genomic DNA";clone_lib="CML cosmid";clone="15.1";db_xref="taxon:9606"
X65921	EMBL	mRNA	408	504	0.000	+	.	ID="X65921.2";featflags="0x100";gene="fau 1"
X65921	EMBL	mRNA	774	856	0.000	+	.	ID="X65921.2";featflags="0x104"
X65921	EMBL	mRNA	951	1095	0.000	+	.	ID="X65921.2";featflags="0x104"
X65921	EMBL	mRNA	1557	1612	0.000	+	.	ID="X65921.2";featflags="0x104"
X65921	EMBL	mRNA	1787	1912	0.000	+	.	ID="X65921.2";featflags="0x106"
X65921	EMBL	exon	408	504	0.000	+	.	ID="X65921.3";number=1
X65921	EMBL	intron	505	773	0.000	+	.	ID="X65921.4";number=1
X65921	EMBL	exon	774	856	0.000	+	.	ID="X65921.5";number=2
X65921	EMBL	CDS	782	856	0.000	+	0	ID="X65921.6";featflags="0x100";gene="fau 1";db_xref="GDB:135476";db_xref="GOA:P62861";db_xref="HGNC:3597";db_xref="HSSP:1GJZ";db_xref="InterPro:IPR006846";db_xref="UniProtKB/Swiss-Prot:P35544";protein_id="CAA46714.1";translation="MQLFVRAQELHTFEVTGQETVAQIKAHVASLEGIAPEDQVVLLAGAPLEDEATLGQCGVEALTTLEVAGRMLGGKVHGSLARAGKVRGQTPKVAKQEKKKKKTGRAKRRMQYNRRFVNVVPTFGKKKGPNANS"
X65921	EMBL	CDS	951	1095	0.000	+	0	ID="X65921.6";featflags="0x104"
X65921	EMBL	CDS	1557	1612	0.000	+	0	ID="X65921.6";featflags="0x104"
X65921	EMBL	CDS	1787	1912	0.000	+	0	ID="X65921.6";featflags="0x104"
X65921	EMBL	intron	857	950	0.000	+	.	ID="X65921.7";number=2
X65921	EMBL	exon	951	1095	0.000	+	.	ID="X65921.8";number=3
X65921	EMBL	intron	1096	1556	0.000	+	.	ID="X65921.9";number=3
X65921	EMBL	exon	1557	1612	0.000	+	.	ID="X65921.10";number=4
X65921	EMBL	intron	1613	1786	0.000	+	.	ID="X65921.11";number=4
X65921	EMBL	exon	1787	1912	0.000	+	.	ID="X65921.12";featflags="0x2";number=5
X65921	EMBL	polyA_signal_sequence	1938	1943	0.000	+	.	ID="X65921.13"

A.2.4. GFF2

The older General Feature Format version 2 (GFF2) is a format developed at the Sanger Institute for describing genes and other features associated with DNA, RNA and protein sequences. GFF2 describes each feature with a name, start position and end position (or "NSE") along with extended annotation. The format also provides limited support for pairs of features. GFF2 format is normally used to hold pure feature information only, but can hold the sequence as part of the structured header. A complete specification of the format is available at:

http://www.sanger.ac.uk/Software/formats/GFF/GFF_Spec.shtml
##gff-version 2.0
##date 2009-07-30
##Type DNA X65921
X65921	EMBL	source	1	2016	0.000	+	.	Sequence "X65921.1" ; organism "Homo sapiens" ; mol_type "genomic DNA" ; clone_lib "CML cosmid" ; clone "15.1" ; db_xref "taxon:9606"
X65921	EMBL	mRNA	408	504	0.000	+	.	Sequence "X65921.2" ; FeatFlags "0x100" ; gene "fau 1"
X65921	EMBL	mRNA	774	856	0.000	+	.	Sequence "X65921.2" ; FeatFlags "0x104"
X65921	EMBL	mRNA	951	1095	0.000	+	.	Sequence "X65921.2" ; FeatFlags "0x104"
X65921	EMBL	mRNA	1557	1612	0.000	+	.	Sequence "X65921.2" ; FeatFlags "0x104"
X65921	EMBL	mRNA	1787	1912	0.000	+	.	Sequence "X65921.2" ; FeatFlags "0x106"
X65921	EMBL	exon	408	504	0.000	+	.	Sequence "X65921.3" ; number 1
X65921	EMBL	intron	505	773	0.000	+	.	Sequence "X65921.4" ; number 1
X65921	EMBL	exon	774	856	0.000	+	.	Sequence "X65921.5" ; number 2
X65921	EMBL	CDS	782	856	0.000	+	.	Sequence "X65921.6" ; FeatFlags "0x100" ; gene "fau 1" ; db_xref "GDB:135476" ; db_xref "GOA:P62861" ; db_xref "HGNC:3597" ; db_xref "HSSP:1GJZ" ; db_xref "InterPro:IPR006846" ; db_xref "UniProtKB/Swiss-Prot:P35544" ; protein_id "CAA46714.1" ; translation "MQLFVRAQELHTFEVTGQETVAQIKAHVASLEGIAPEDQVVLLAGAPLEDEATLGQCGVEALTTLEVAGRMLGGKVHGSLARAGKVRGQTPKVAKQEKKKKKTGRAKRRMQYNRRFVNVVPTFGKKKGPNANS"
X65921	EMBL	CDS	951	1095	0.000	+	.	Sequence "X65921.6" ; FeatFlags "0x104"
X65921	EMBL	CDS	1557	1612	0.000	+	.	Sequence "X65921.6" ; FeatFlags "0x104"
X65921	EMBL	CDS	1787	1912	0.000	+	.	Sequence "X65921.6" ; FeatFlags "0x104"
X65921	EMBL	intron	857	950	0.000	+	.	Sequence "X65921.7" ; number 2
X65921	EMBL	exon	951	1095	0.000	+	.	Sequence "X65921.8" ; number 3
X65921	EMBL	intron	1096	1556	0.000	+	.	Sequence "X65921.9" ; number 3
X65921	EMBL	exon	1557	1612	0.000	+	.	Sequence "X65921.10" ; number 4
X65921	EMBL	intron	1613	1786	0.000	+	.	Sequence "X65921.11" ; number 4
X65921	EMBL	exon	1787	1912	0.000	+	.	Sequence "X65921.12" ; FeatFlags "0x2" ; number 5
X65921	EMBL	polyA_signal_sequence	1938	1943	0.000	+	.	Sequence "X65921.13"

A.2.5. PIR/NBRF

The PIR / NBRF feature format is that used in the PIR database, an integrated protein informatics resource for genomic and proteomic research. For further information see:

http://pir.georgetown.edu/pirwww/
F;1/Modified site: Removed, cleaved_initiator_methionine
F;2-269/Product: Aquaporin-1 PRO_0000063920
F;2-7/Region: Cytoplasmic, extramembrane
F;8-36/Region: Helix 1, transmembrane
F;37-48/Region: Extracellular, extramembrane
F;49-66/Region: Helix 2, transmembrane
F;67-70/Region: Cytoplasmic, extramembrane
F;71-76/Region: In membrane, extramembrane
F;77-84/Region: Helix B, transmembrane
F;85-94/Region: Cytoplasmic, extramembrane
F;95-115/Region: Helix 3, transmembrane
F;116-136/Region: Extracellular, extramembrane
F;137-155/Region: Helix 4, transmembrane
F;156-166/Region: Cytoplasmic, extramembrane
F;167-183/Region: Helix 5, transmembrane
F;184-186/Region: Extracellular, extramembrane
F;187-192/Region: In membrane, extramembrane
F;193-200/Region: Helix E, transmembrane
F;201-207/Region: Extracellular, extramembrane
F;208-228/Region: Helix 6, transmembrane
F;229-269/Region: Cytoplasmic, extramembrane
F;76-78/Region: NPA 1, polypeptide_motif
F;192-194/Region: NPA 2, polypeptide_motif
F;159-162/Region: Poly-Arg, computationally_biased_region
F;56/Region: Substrate discrimination, polypeptide_region
F;180/Region: Substrate discrimination, polypeptide_region
F;189/Region: Hg(2+)-sensitive residue, polypeptide_region
F;195/Region: Substrate discrimination, polypeptide_region
F;246/Modified site: Phosphothreonine, protein_modification_categorized_by_chemical_process #By similarity
F;247/Modified site: Phosphoserine, protein_modification_categorized_by_chemical_process #By similarity
F;262/Modified site: Phosphoserine, protein_modification_categorized_by_chemical_process
F;42/Modified site: N-linked, glycosylated_residue #GlcNAc...
F;205/Modified site: N-linked (GlcNAc...), glycosylated_residue #Potential
F;38/Region: P -> L (in Co(A-B-) antigen; non functional AQP1; red cells show low osmotic water permeability), natural_variant VAR_013279
F;45/Region: A -> V (in Co(A-B+) antigen; dbSNP:rs28362692), natural_variant VAR_004400
F;165/Region: G -> D, natural_variant VAR_022318 #in dbSNP:rs28362731
F;45/Region: A -> T, sequence_conflict #in Ref. 7; AAH22486
F;8-35/Region: alpha_helix
F;37-42/Region: beta_strand
F;48-65/Region: alpha_helix
F;68-71/Region: beta_strand
F;76-83/Region: alpha_helix
F;94-114/Region: alpha_helix
F;119-122/Region: turn
F;132-135/Region: beta_strand
F;136-154/Region: alpha_helix
F;166-182/Region: alpha_helix
F;183-185/Region: turn
F;192-199/Region: alpha_helix
F;207-227/Region: alpha_helix

A.2.6. SwissProt

SwissProt feature format. UniProtKB / SwissProt is a curated protein sequence database with high quality annotation on the protein function, domain structure, post-translational modifications, variants, etc. It has a minimal level of redundancy and high level of integration with other databases. For further information see:

http://expasy.org/sprot/
http://expasy.org/sprot/userman.html#FT_line.
http://expasy.org/sprot/userman.html#Appendix_A (feature table keys)
FT   INIT_MET      1      1       Removed.
FT   CHAIN         2    269       Aquaporin-1.
FT                                /FTId=PRO_0000063920.
FT   TOPO_DOM      2      7       Cytoplasmic.
FT   TRANSMEM      8     36       Helix 1.
FT   TOPO_DOM     37     48       Extracellular.
FT   TRANSMEM     49     66       Helix 2.
FT   TOPO_DOM     67     70       Cytoplasmic.
FT   TOPO_DOM     71     76       In membrane.
FT   TRANSMEM     77     84       Helix B.
FT   TOPO_DOM     85     94       Cytoplasmic.
FT   TRANSMEM     95    115       Helix 3.
FT   TOPO_DOM    116    136       Extracellular.
FT   TRANSMEM    137    155       Helix 4.
FT   TOPO_DOM    156    166       Cytoplasmic.
FT   TRANSMEM    167    183       Helix 5.
FT   TOPO_DOM    184    186       Extracellular.
FT   TOPO_DOM    187    192       In membrane.
FT   TRANSMEM    193    200       Helix E.
FT   TOPO_DOM    201    207       Extracellular.
FT   TRANSMEM    208    228       Helix 6.
FT   TOPO_DOM    229    269       Cytoplasmic.
FT   MOTIF        76     78       NPA 1.
FT   MOTIF       192    194       NPA 2.
FT   COMPBIAS    159    162       Poly-Arg.
FT   SITE         56     56       Substrate discrimination.
FT   SITE        180    180       Substrate discrimination.
FT   SITE        189    189       Hg(2+)-sensitive residue.
FT   SITE        195    195       Substrate discrimination.
FT   MOD_RES     246    246       Phosphothreonine (By similarity).
FT   MOD_RES     247    247       Phosphoserine (By similarity).
FT   MOD_RES     262    262       Phosphoserine.
FT   CARBOHYD     42     42       N-linked (GlcNAc...).
FT   CARBOHYD    205    205       N-linked (GlcNAc...) (Potential).
FT   VARIANT      38     38       P -> L (in Co(A-B-) antigen; non
FT                                functional AQP1; red cells show low
FT                                osmotic water permeability).
FT                                /FTId=VAR_013279.
FT   VARIANT      45     45       A -> V (in Co(A-B+) antigen;
FT                                dbSNP:rs28362692).
FT                                /FTId=VAR_004400.
FT   VARIANT     165    165       G -> D (in dbSNP:rs28362731).
FT                                /FTId=VAR_022318.
FT   CONFLICT     45     45       A -> T (in Ref. 7; AAH22486).
FT   HELIX         8     35
FT   STRAND       37     42
FT   HELIX        48     65
FT   STRAND       68     71
FT   HELIX        76     83
FT   HELIX        94    114
FT   TURN        119    122
FT   STRAND      132    135
FT   HELIX       136    154
FT   HELIX       166    182
FT   TURN        183    185
FT   HELIX       192    199
FT   HELIX       207    227