eiprscan |
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% eiprscan -nocrc -goterms -iprlookup Motif detection Input protein sequence set: iprtest.seq Applications to use all : all blastprodom : blastprodom coils : coils gene3d : gene3d hmmpanther : hmmpanther hmmpir : hmmpir hmmpfam : hmmpfam hmmsmart : hmmsmart hmmtigr : hmmtigr fprintscan : fprintscan scanregexp : scanregexp profilescan : profilescan superfamily : superfamily seg : seg signalp : signalp tmhmm : tmhmm Application(s) to use [all]: Output Formats raw : Raw txt : Text html : Html xml : Xml ebixml : EBIxml gff : GFF Format to use [xml]: raw EIPRSCAN program output file [iprtest.eiprscan]: SUBMITTED-iprscan-20110715-12345678 |
Go to the input files for this example
Go to the output files for this example
Motif detection Version: EMBOSS:6.4.0.0 Standard (Mandatory) qualifiers: [-sequence] seqset Protein sequence set filename and optional format, or reference (input USA) -appl menu [all] Application(s) to use (Values: all (all); blastprodom (blastprodom); coils (coils); gene3d (gene3d); hmmpanther (hmmpanther); hmmpir (hmmpir); hmmpfam (hmmpfam); hmmsmart (hmmsmart); hmmtigr (hmmtigr); fprintscan (fprintscan); scanregexp (scanregexp); profilescan (profilescan); superfamily (superfamily); seg (seg); signalp (signalp); tmhmm (tmhmm)) -format menu [xml] Format to use (Values: raw (Raw); txt (Text); html (Html); xml (Xml); ebixml (EBIxml); gff (GFF)) [-outfile] outfile [*.eiprscan] EIPRSCAN program output file Additional (Optional) qualifiers (* if not always prompted): -email string Submitter email address (Any string) -trtable menu [0] Genetic codes used for translation (Values: 0 (Standard); 1 (Standard (with alternative initiation codons)); 2 (Vertebrate Mitochondrial); 3 (Yeast Mitochondrial); 4 (Mold, Protozoan, Coelenterate Mitochondrial and Mycoplasma/Spiroplasma); 5 (Invertebrate Mitochondrial); 6 (Ciliate Macronuclear and Dasycladacean); 9 (Echinoderm Mitochondrial); 10 (Euplotid Nuclear); 11 (Bacterial); 12 (Alternative Yeast Nuclear); 13 (Ascidian Mitochondrial); 14 (Flatworm Mitochondrial); 15 (Blepharisma Macronuclear); 16 (Chlorophycean Mitochondrial); 21 (Trematode Mitochondrial); 22 (Scenedesmus obliquus); 23 (Thraustochytrium Mitochondrial)) -trlen integer [1] Minimum size of Open Reading Frames (ORFs) in the translations. (Integer 1 or more) -iprlookup boolean [N] Turn on InterPro lookup for results * -goterms boolean [N] Show GO terms in InterPro lookup -[no]crc boolean [Y] Perform CRC64 check -altjobs boolean [N] Launch jobs alternately (chunk after chunk) Advanced (Unprompted) qualifiers: (none) Associated qualifiers: "-sequence" associated qualifiers -sbegin1 integer Start of each sequence to be used -send1 integer End of each sequence to be used -sreverse1 boolean Reverse (if DNA) -sask1 boolean Ask for begin/end/reverse -snucleotide1 boolean Sequence is nucleotide -sprotein1 boolean Sequence is protein -slower1 boolean Make lower case -supper1 boolean Make upper case -sformat1 string Input sequence format -sdbname1 string Database name -sid1 string Entryname -ufo1 string UFO features -fformat1 string Features format -fopenfile1 string Features file name "-outfile" associated qualifiers -odirectory2 string Output directory General qualifiers: -auto boolean Turn off prompts -stdout boolean Write first file to standard output -filter boolean Read first file from standard input, write first file to standard output -options boolean Prompt for standard and additional values -debug boolean Write debug output to program.dbg -verbose boolean Report some/full command line options -help boolean Report command line options and exit. More information on associated and general qualifiers can be found with -help -verbose -warning boolean Report warnings -error boolean Report errors -fatal boolean Report fatal errors -die boolean Report dying program messages -version boolean Report version number and exit |
Qualifier | Type | Description | Allowed values | Default | ||||||||||||||||||||||||||||||||||||
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Standard (Mandatory) qualifiers | ||||||||||||||||||||||||||||||||||||||||
[-sequence] (Parameter 1) |
seqset | Protein sequence set filename and optional format, or reference (input USA) | Readable set of sequences | Required | ||||||||||||||||||||||||||||||||||||
-appl | list | Application(s) to use |
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all | ||||||||||||||||||||||||||||||||||||
-format | list | Format to use |
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xml | ||||||||||||||||||||||||||||||||||||
[-outfile] (Parameter 2) |
outfile | EIPRSCAN program output file | Output file | <*>.eiprscan | ||||||||||||||||||||||||||||||||||||
Additional (Optional) qualifiers | ||||||||||||||||||||||||||||||||||||||||
string | Submitter email address | Any string | ||||||||||||||||||||||||||||||||||||||
-trtable | list | Genetic codes used for translation |
|
0 | ||||||||||||||||||||||||||||||||||||
-trlen | integer | Minimum size of Open Reading Frames (ORFs) in the translations. | Integer 1 or more | 1 | ||||||||||||||||||||||||||||||||||||
-iprlookup | boolean | Turn on InterPro lookup for results | Boolean value Yes/No | No | ||||||||||||||||||||||||||||||||||||
-goterms | boolean | Show GO terms in InterPro lookup | Boolean value Yes/No | No | ||||||||||||||||||||||||||||||||||||
-[no]crc | boolean | Perform CRC64 check | Boolean value Yes/No | Yes | ||||||||||||||||||||||||||||||||||||
-altjobs | boolean | Launch jobs alternately (chunk after chunk) | Boolean value Yes/No | No | ||||||||||||||||||||||||||||||||||||
Advanced (Unprompted) qualifiers | ||||||||||||||||||||||||||||||||||||||||
(none) | ||||||||||||||||||||||||||||||||||||||||
Associated qualifiers | ||||||||||||||||||||||||||||||||||||||||
"-sequence" associated seqset qualifiers | ||||||||||||||||||||||||||||||||||||||||
-sbegin1 -sbegin_sequence |
integer | Start of each sequence to be used | Any integer value | 0 | ||||||||||||||||||||||||||||||||||||
-send1 -send_sequence |
integer | End of each sequence to be used | Any integer value | 0 | ||||||||||||||||||||||||||||||||||||
-sreverse1 -sreverse_sequence |
boolean | Reverse (if DNA) | Boolean value Yes/No | N | ||||||||||||||||||||||||||||||||||||
-sask1 -sask_sequence |
boolean | Ask for begin/end/reverse | Boolean value Yes/No | N | ||||||||||||||||||||||||||||||||||||
-snucleotide1 -snucleotide_sequence |
boolean | Sequence is nucleotide | Boolean value Yes/No | N | ||||||||||||||||||||||||||||||||||||
-sprotein1 -sprotein_sequence |
boolean | Sequence is protein | Boolean value Yes/No | N | ||||||||||||||||||||||||||||||||||||
-slower1 -slower_sequence |
boolean | Make lower case | Boolean value Yes/No | N | ||||||||||||||||||||||||||||||||||||
-supper1 -supper_sequence |
boolean | Make upper case | Boolean value Yes/No | N | ||||||||||||||||||||||||||||||||||||
-sformat1 -sformat_sequence |
string | Input sequence format | Any string | |||||||||||||||||||||||||||||||||||||
-sdbname1 -sdbname_sequence |
string | Database name | Any string | |||||||||||||||||||||||||||||||||||||
-sid1 -sid_sequence |
string | Entryname | Any string | |||||||||||||||||||||||||||||||||||||
-ufo1 -ufo_sequence |
string | UFO features | Any string | |||||||||||||||||||||||||||||||||||||
-fformat1 -fformat_sequence |
string | Features format | Any string | |||||||||||||||||||||||||||||||||||||
-fopenfile1 -fopenfile_sequence |
string | Features file name | Any string | |||||||||||||||||||||||||||||||||||||
"-outfile" associated outfile qualifiers | ||||||||||||||||||||||||||||||||||||||||
-odirectory2 -odirectory_outfile |
string | Output directory | Any string | |||||||||||||||||||||||||||||||||||||
General qualifiers | ||||||||||||||||||||||||||||||||||||||||
-auto | boolean | Turn off prompts | Boolean value Yes/No | N | ||||||||||||||||||||||||||||||||||||
-stdout | boolean | Write first file to standard output | Boolean value Yes/No | N | ||||||||||||||||||||||||||||||||||||
-filter | boolean | Read first file from standard input, write first file to standard output | Boolean value Yes/No | N | ||||||||||||||||||||||||||||||||||||
-options | boolean | Prompt for standard and additional values | Boolean value Yes/No | N | ||||||||||||||||||||||||||||||||||||
-debug | boolean | Write debug output to program.dbg | Boolean value Yes/No | N | ||||||||||||||||||||||||||||||||||||
-verbose | boolean | Report some/full command line options | Boolean value Yes/No | Y | ||||||||||||||||||||||||||||||||||||
-help | boolean | Report command line options and exit. More information on associated and general qualifiers can be found with -help -verbose | Boolean value Yes/No | N | ||||||||||||||||||||||||||||||||||||
-warning | boolean | Report warnings | Boolean value Yes/No | Y | ||||||||||||||||||||||||||||||||||||
-error | boolean | Report errors | Boolean value Yes/No | Y | ||||||||||||||||||||||||||||||||||||
-fatal | boolean | Report fatal errors | Boolean value Yes/No | Y | ||||||||||||||||||||||||||||||||||||
-die | boolean | Report dying program messages | Boolean value Yes/No | Y | ||||||||||||||||||||||||||||||||||||
-version | boolean | Report version number and exit | Boolean value Yes/No | N |
>RS16_ECOLI MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIASEKEEGTRLDLDRIAHW VGQGATISDRVAALIKEVNKAA >Q9RHD9 XPKLEEGVEGLVHVSEMDWTNKNIHPSKVVQVGDEVEVQVLDIDEERRRISLGIKQCKSN PWEDFSSQFNKGDRISGSIKSITDFGIFIGLDGGIDGLVHLSDISWNEVGEEAVRRFKKG DELETVILSVDPERERISLGIKQLEDDPFSNYASLHEKGSIVRGTVKEVDAKGAVISLGD DIEGILKASEISRDRVEDARNVLKEGEEVEAKIISIDRKSRVISLSVKSKDVDDEKDAMK ELRKQEVESAGPTTIGDLIRAQMENQG >Y902_MYCTU Q10560 PROBABLE SENSOR-LIKE HISTIDINE KINASE RV0902C (EC 2.7.3.-). MNILSRIFARTPSLRTRVVVATAIGAAIPVLIVGTVVWVGITNDRKERLDRRLDEAAGFA IPFVPRGLDEIPRSPNDQDALITVRRGNVIKSNSDITLPKLQDDYADTYVRGVRYRVRTV EIPGPEPTSVAVGATYDATVAETNNLHRRVLLICTFAIGAAAVFAWLLAAFAVRPFKQLA EQTRSIDAGDEAPRVEVHGASEAIEIAEAMRGMLQRIWNEQNRTKEALASARDFAAVSSH ELRTPLTAMRTNLEVLSTLDLPDDQRKEVLNDVIRTQSRIEATLSALERLAQGELSTSDD HVPVDITDLLDRAAHDAARIYPDLDVSLVPSPTCIIVGLPAGLRLAVDNAIANAVKHGGA TLVQLSAVSSRAGVEIAIDDNGSGVPEGERQVVFERFSRGSTASHSGSGLGLALVAQQAQ LHGGTASLENSPLGGARLVLRLPGPS |
Q9RHD9 D44DAE8C544CB7C1 267 Coil coil coiled-coil 225 246 NA ? 11-Jul-2011 NULL NULL Q9RHD9 D44DAE8C544CB7C1 267 Gene3D G3DSA:2.40.50.140 no description 4 65 2.2e-21 T 11-Jul-2011 IPR012340 Nucleic acid-binding, OB-fold Q9RHD9 D44DAE8C544CB7C1 267 Gene3D G3DSA:2.40.50.140 no description 66 146 5.8e-25 T 11-Jul-2011 IPR012340 Nucleic acid-binding, OB-fold Q9RHD9 D44DAE8C544CB7C1 267 Gene3D G3DSA:2.40.50.140 no description 147 228 1.6e-21 T 11-Jul-2011 IPR012340 Nucleic acid-binding, OB-fold RS16_ECOLI F94D07049A6D489D 82 Gene3D G3DSA:3.30.1320.10 no description 1 79 1.1e-31 T 11-Jul-2011 IPR023803 Ribosomal protein S16 domain Y902_MYCTU CD84A335CCFFE6D7 446 Gene3D G3DSA:1.10.287.240 no description 222 287 2.4e-08 T 11-Jul-2011 NULL NULL Y902_MYCTU CD84A335CCFFE6D7 446 Gene3D G3DSA:3.30.565.10 no description 302 444 5.2e-26 T 11-Jul-2011 IPR003594 ATPase-like, ATP-binding domain Molecular Function: ATP binding (GO:0005524) Y902_MYCTU CD84A335CCFFE6D7 446 HMMPanther PTHR24423 FAMILY NOT NAMED 27 443 6.7e-36 T 11-Jul-2011 NULL NULL RS16_ECOLI F94D07049A6D489D 82 HMMPanther PTHR12919:SF12 SUBFAMILY NOT NAMED 1 77 5.2e-50 T 11-Jul-2011 NULL NULL RS16_ECOLI F94D07049A6D489D 82 HMMPanther PTHR12919 FAMILY NOT NAMED 1 77 5.2e-50 T 11-Jul-2011 IPR000307 Ribosomal protein S16 Molecular Function: structural constituent of ribosome (GO:0003735), Cellular Component: intracellular (GO:0005622), Cellular Component: ribosome (GO:0005840), Biological Process: translation (GO:0006412) Q9RHD9 D44DAE8C544CB7C1 267 HMMPanther PTHR10724 S1 RNA-BINDING DOMAIN-CONTAINING PROTEIN 1 4 156 2.4e-69 T 11-Jul-2011 NULL NULL RS16_ECOLI F94D07049A6D489D 82 HMMPfam PF00886 Ribosomal_S16 8 68 8.7e-26 T 11-Jul-2011 IPR000307 Ribosomal protein S16 Molecular Function: structural constituent of ribosome (GO:0003735), Cellular Component: intracellular (GO:0005622), Cellular Component: ribosome (GO:0005840), Biological Process: translation (GO:0006412) Q9RHD9 D44DAE8C544CB7C1 267 HMMPfam PF00575 S1 4 55 1.5e-14 T 11-Jul-2011 IPR003029 Ribosomal protein S1, RNA-binding domain Molecular Function: RNA binding (GO:0003723) Q9RHD9 D44DAE8C544CB7C1 267 HMMPfam PF00575 S1 69 142 1.8e-18 T 11-Jul-2011 IPR003029 Ribosomal protein S1, RNA-binding domain Molecular Function: RNA binding (GO:0003723) Q9RHD9 D44DAE8C544CB7C1 267 HMMPfam PF00575 S1 156 228 4.7e-19 T 11-Jul-2011 IPR003029 Ribosomal protein S1, RNA-binding domain Molecular Function: RNA binding (GO:0003723) Y902_MYCTU CD84A335CCFFE6D7 446 HMMPfam PF02518 HATPase_c 340 443 4.6e-18 T 11-Jul-2011 IPR003594 ATPase-like, ATP-binding domain Molecular Function: ATP binding (GO:0005524) Y902_MYCTU CD84A335CCFFE6D7 446 HMMPfam PF00512 HisKA 232 293 3.2e-11 T 11-Jul-2011 IPR003661 Signal transduction histidine kinase, subgroup 1, dimerisation/phosphoacceptor domain Molecular Function: two-component sensor activity (GO:0000155), Biological Process: signal transduction (GO:0007165), Cellular Component: membrane (GO:0016020) Y902_MYCTU CD84A335CCFFE6D7 446 HMMPfam PF00672 HAMP 151 217 2.1e-10 T 11-Jul-2011 IPR003660 HAMP linker domain Molecular Function: signal transducer activity (GO:0004871), Biological Process: signal transduction (GO:0007165), Cellular Component: integral to membrane (GO:0016021) Q9RHD9 D44DAE8C544CB7C1 267 HMMSmart SM00316 no description 3 55 1.2e-06 T 11-Jul-2011 IPR022967 RNA-binding domain, S1 Q9RHD9 D44DAE8C544CB7C1 267 HMMSmart SM00316 no description 70 142 1.4e-19 T 11-Jul-2011 IPR022967 RNA-binding domain, S1 Q9RHD9 D44DAE8C544CB7C1 267 HMMSmart SM00316 no description 157 228 2.6e-21 T 11-Jul-2011 IPR022967 RNA-binding domain, S1 Y902_MYCTU CD84A335CCFFE6D7 446 HMMSmart SM00304 no description 170 222 3.1e-06 T 11-Jul-2011 IPR003660 HAMP linker domain Molecular Function: signal transducer activity (GO:0004871), Biological Process: signal transduction (GO:0007165), Cellular Component: integral to membrane (GO:0016021) Y902_MYCTU CD84A335CCFFE6D7 446 HMMSmart SM00388 no description 230 296 2.4e-12 T 11-Jul-2011 IPR003661 Signal transduction histidine kinase, subgroup 1, dimerisation/phosphoacceptor domain Molecular Function: two-component sensor activity (GO:0000155), Biological Process: signal transduction (GO:0007165), Cellular Component: membrane (GO:0016020) Y902_MYCTU CD84A335CCFFE6D7 446 HMMSmart SM00387 no description 338 446 5e-24 T 11-Jul-2011 IPR003594 ATPase-like, ATP-binding domain Molecular Function: ATP binding (GO:0005524) RS16_ECOLI F94D07049A6D489D 82 HMMTigr TIGR00002 S16: ribosomal protein S16 2 78 1.1e-28 T 11-Jul-2011 IPR000307 Ribosomal protein S16 Molecular Function: structural constituent of ribosome (GO:0003735), Cellular Component: intracellular (GO:0005622), Cellular Component: ribosome (GO:0005840), Biological Process: translation (GO:0006412) Q9RHD9 D44DAE8C544CB7C1 267 FPrintScan PR00681 RIBOSOMALS1 6 27 7.9e-17 T 11-Jul-2011 IPR000110 Ribosomal protein S1 Molecular Function: RNA binding (GO:0003723), Molecular Function: structural constituent of ribosome (GO:0003735), Cellular Component: ribosome (GO:0005840), Biological Process: translation (GO:0006412) Q9RHD9 D44DAE8C544CB7C1 267 FPrintScan PR00681 RIBOSOMALS1 85 104 7.9e-17 T 11-Jul-2011 IPR000110 Ribosomal protein S1 Molecular Function: RNA binding (GO:0003723), Molecular Function: structural constituent of ribosome (GO:0003735), Cellular Component: ribosome (GO:0005840), Biological Process: translation (GO:0006412) Q9RHD9 D44DAE8C544CB7C1 267 FPrintScan PR00681 RIBOSOMALS1 125 143 7.9e-17 T 11-Jul-2011 IPR000110 Ribosomal protein S1 Molecular Function: RNA binding (GO:0003723), Molecular Function: structural constituent of ribosome (GO:0003735), Cellular Component: ribosome (GO:0005840), Biological Process: translation (GO:0006412) Y902_MYCTU CD84A335CCFFE6D7 446 FPrintScan PR00344 BCTRLSENSOR 374 388 1.1e-11 T 11-Jul-2011 IPR004358 Signal transduction histidine kinase-related protein, C-terminal Biological Process: phosphorylation (GO:0016310), Molecular Function: transferase activity, transferring phosphorus-containing groups (GO:0016772) Y902_MYCTU CD84A335CCFFE6D7 446 FPrintScan PR00344 BCTRLSENSOR 392 402 1.1e-11 T 11-Jul-2011 IPR004358 Signal transduction histidine kinase-related protein, C-terminal Biological Process: phosphorylation (GO:0016310), Molecular Function: transferase activity, transferring phosphorus-containing groups (GO:0016772) Y902_MYCTU CD84A335CCFFE6D7 446 FPrintScan PR00344 BCTRLSENSOR 406 424 1.1e-11 T 11-Jul-2011 IPR004358 Signal transduction histidine kinase-related protein, C-terminal Biological Process: phosphorylation (GO:0016310), Molecular Function: transferase activity, transferring phosphorus-containing groups (GO:0016772) Y902_MYCTU CD84A335CCFFE6D7 446 FPrintScan PR00344 BCTRLSENSOR 430 443 1.1e-11 T 11-Jul-2011 IPR004358 Signal transduction histidine kinase-related protein, C-terminal Biological Process: phosphorylation (GO:0016310), Molecular Function: transferase activity, transferring phosphorus-containing groups (GO:0016772) RS16_ECOLI F94D07049A6D489D 82 superfamily SSF54565 Ribosomal protein S16 1 79 5.3e-27 T 11-Jul-2011 IPR023803 Ribosomal protein S16 domain Q9RHD9 D44DAE8C544CB7C1 267 superfamily SSF50249 Nucleic acid-binding proteins 137 254 1.9e-29 T 11-Jul-2011 IPR016027 Nucleic acid-binding, OB-fold-like Q9RHD9 D44DAE8C544CB7C1 267 superfamily SSF50249 Nucleic acid-binding proteins 58 143 8.5e-23 T 11-Jul-2011 IPR016027 Nucleic acid-binding, OB-fold-like Q9RHD9 D44DAE8C544CB7C1 267 superfamily SSF50249 Nucleic acid-binding proteins 3 68 2.5e-15 T 11-Jul-2011 IPR016027 Nucleic acid-binding, OB-fold-like Y902_MYCTU CD84A335CCFFE6D7 446 superfamily SSF55874 ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase 299 446 2.1e-33 T 11-Jul-2011 IPR003594 ATPase-like, ATP-binding domain Molecular Function: ATP binding (GO:0005524) Y902_MYCTU CD84A335CCFFE6D7 446 superfamily SSF47384 Homodimeric domain of signal transducing histidine kinase 212 298 5.5e-12 T 11-Jul-2011 IPR009082 Signal transduction histidine kinase, homodimeric Molecular Function: signal transducer activity (GO:0004871), Biological Process: signal transduction (GO:0007165) Q9RHD9 D44DAE8C544CB7C1 267 Seg seg seg 29 40 NA ? 11-Jul-2011 NULL NULL Q9RHD9 D44DAE8C544CB7C1 267 Seg seg seg 84 98 NA ? 11-Jul-2011 NULL NULL Q9RHD9 D44DAE8C544CB7C1 267 Seg seg seg 222 237 NA ? 11-Jul-2011 NULL NULL Y902_MYCTU CD84A335CCFFE6D7 446 Seg seg seg 44 55 NA ? 11-Jul-2011 NULL NULL Y902_MYCTU CD84A335CCFFE6D7 446 Seg seg seg 108 120 NA ? 11-Jul-2011 NULL NULL Y902_MYCTU CD84A335CCFFE6D7 446 Seg seg seg 160 173 NA ? 11-Jul-2011 NULL NULL Y902_MYCTU CD84A335CCFFE6D7 446 Seg seg seg 308 319 NA ? 11-Jul-2011 NULL NULL Y902_MYCTU CD84A335CCFFE6D7 446 Seg seg seg 400 424 NA ? 11-Jul-2011 NULL NULL |
See Zdobnov E.M. and Apweiler R. "InterProScan - an integration platform for the signature-recognition methods in InterPro" Bioinformatics, 2001, 17(9): p. 847-8.
See InterPro documentation available at: http://www.ebi.ac.uk/interpro/
Program name | Description |
---|---|
antigenic | Finds antigenic sites in proteins |
elipop | Prediction of lipoproteins |
emast | Motif detection |
ememe | Multiple EM for Motif Elicitation |
ememetext | Multiple EM for Motif Elicitation. Text file only |
epestfind | Finds PEST motifs as potential proteolytic cleavage sites |
fuzzpro | Search for patterns in protein sequences |
fuzztran | Search for patterns in protein sequences (translated) |
omeme | Motif detection |
patmatdb | Searches protein sequences with a sequence motif |
patmatmotifs | Scan a protein sequence with motifs from the PROSITE database |
preg | Regular expression search of protein sequence(s) |
pscan | Scans protein sequence(s) with fingerprints from the PRINTS database |
sigcleave | Reports on signal cleavage sites in a protein sequence |
The original iprscan application must be installed and configured to use this wrapper.
Please report all bugs to the EMBOSS bug team (emboss-bug © emboss.open-bio.org) not to the original author.